Figure 2.

Structural interactions between RA14 CDR3 loops and NLV peptide/A2. The crystal structure of the RA14 TCR complexed with NLV/A2 (PDB code 3GSN) was used to guide library design. The A2 surface is shown in gray space-fill, the NLV peptide surface in pink space-fill, and the RA14 structure in purple ribbon. The residues comprising the CDR3α (a) and CDR3β (b) loops are listed, with those targeted for mutagenesis highlighted in red in the text and in the structure. Boxed residues form direct pMHC contacts in the WT crystal as reported previously (14).