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. 2019 Feb 19;294(15):5945–5955. doi: 10.1074/jbc.RA118.006029

Figure 3.

Figure 3.

GADD34 up-regulates MCL-1 through TRAF6 and ERK1/2. A, HepG2 and SMMC-7721 cells were transfected with the EGFP-tagged GADD34 expression plasmid or EGFP vector, followed by Western blotting analysis of p-ERK1/2 (Thr202/Tyr204), ERK1/2, p-Akt (Ser473), Akt, and GADD34. B, HepG2 and SMMC-7721 cells were transfected with siControl or siGADD34, followed by Western blotting analysis of p-ERK1/2 (Thr202/Tyr204), ERK1/2, p-Akt (Ser473), Akt, and GADD34. C, HepG2 and SMMC-7721 cells were transfected with siControl or siGADD34#2, followed by Western blotting analysis of p-ERK1/2 (Thr202/Tyr204), ERK1/2, p-Akt (Ser473), Akt, and GADD34. D, HepG2 and SMMC-7721 cells were transfected with the EGFP-tagged GADD34 expression plasmid or EGFP vector and treated with or without 10 μm of U0126 for 48 h, followed by Western blotting analysis of MCL-1, p-ERK1/2 (Thr202/Tyr204), ERK1/2, and GADD34.