Figure 6.

MCL-1 suppresses the potentiation of TRAIL-induced apoptosis by GADD34 depletion in HepG2 cells. A, HepG2 cells were transfected with siControl or siGADD34, and the MCL-1 expression plasmid or vector, followed by treatment with or without 100 ng/ml of TRAIL for 24 h. The levels of cleaved caspase-7, PARP, GADD34, and MCL-1 were detected by Western blotting. B, HepG2 cells were transfected with siControl or siGADD34 and the MCL-1 expression plasmid or vector followed by treatment with or without 100 ng/ml of TRAIL for 24 h. Cell apoptosis was detected by TUNEL assays. C, the apoptotic rate was plotted. The values represent the means ± S.D. (n = 3). ***, p < 0.001. DAPI, 4′,6′-diamino-2-phenylindole.