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Binding affinities of TKB58, TKB27, and C9B7W to mouse LAG-3. A–C, binding affinities of anti-mouse LAG-3 Abs to mouse LAG-3 protein. LAG-3–EC was immobilized on the biosensor chip, and the association of TKB58 (A), TKB27 (B), and C9B7W (C) at the indicated concentration was monitored by biolayer interferometry. Chips were washed with PBS to analyze the dissociation kinetics; ka, kd, and kD are shown. Data are representative of more than two independent experiments.
