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. 2019 Feb 13;294(15):6017–6026. doi: 10.1074/jbc.RA119.007455

Figure 6.

Figure 6.

EX repeat–dependent inhibition of T cell activation by LAG-3. A and B, EX repeat–dependent partial inhibitory effect of the LAG-3 mutant lacking the FXXL motif. A, amino acid sequences of the IC region of LAG-3 and its mutants. B, DO11.10 cells with LAG-3 and its mutants were stimulated with pOVA-pulsed (1 μm) IIA1.6 cells in the presence of anti-LAG-3–blocking Ab (TKB58) or its isotype control (ctrl Ig). The concentration of IL-2 in the culture supernatant was determined by ELISA. C, different manners of co-stimulation by CD4 and the LAG-3 mutant lacking both the FXXL motif and EX repeat. DO11.10 T cells overexpressing mouse CD4 were stimulated with IIA1.6 cells pulsed with the indicated amount of pOVA in the presence or absence of anti-CD4–blocking Ab (GK1.5). The concentration of IL-2 in the culture supernatant was determined by ELISA. Data are the mean ± S.D. of technical duplicates in one experiment. Data are representative of more than three independent experiments. ND, not detected.