Figure 6.

Cyclin H expression levels and the state of phosphorylation (pThr-315) in HCMV-infected cells. A, validation of the specificity of the phospho-cyclin H antibody by λ-phosphatase assay performed with HeLa cell lysates. B and C, HFF cells were infected with HCMV AD169 and harvested at different time points during infection. Protein levels of immediate early (IE1), early (pUL44), and late viral proteins (MCP) as well as cyclin H were detected by WB analysis using the antibodies as indicated.