Table 3.
Phosphosites of HCMV kinase pUL97
| Phosphositea | Analysis Ib | Analysis IIc | Previously publishedd |
|---|---|---|---|
| Ser-2 | + | ||
| Ser-3 | + | ||
| Ser-11 | + | ||
| Ser-13 | + | ± | + |
| Thr-16 | + | ||
| Thr-18 | + | ||
| Ser-121 | + | + | |
| Ser-133 | ± | + | |
| Thr-134 | + | ||
| Ser-135 | + | + | |
| Ser-136 | ± | + | |
| (Ser-139) | + | ||
| Ser-142 | + | + | |
| Thr-177 | + | + | |
| Ser-180 | + | + | + |
| Ser-183 | + | + | |
| Ser-185 | ± | + | + |
| Ser-187 | ± | + | |
| Thr-190 | + | ||
| Ser-232 | + | + | |
| Ser-235 | + | ||
| Ser-239 | + | + | |
| Tyr-326 | + | ||
| Ser-386 | + | ||
| Thr-482 | + | ||
| Ser-483 | + | ||
| (Thr-504) | ± | ||
| Thr-580 |
a Boldface shows newly detected sites (sites detected only once are shown in parentheses). Detection levels were defined as follows; ±, <75% probability; +, ≥75% probability.
b Samples for analysis I were prepared from HFFs infected with HCMV AD169 for 5 days; pUL97 was immunoprecipitated from total lysates by the use of a pool of two specific polyclonal and monoclonal antibodies.
c Samples for analysis II were prepared from gradient-purified HCMV parental AD169/HB15 or mutant UL97ΔK355 virions, respectively; input material was adjusted by SDS-PAGE separation of the samples followed by Coomassie/silver staining.