Table 4.
Quantities of pUL97–pUL97 self-interaction determined by Western blot densitometry using proteins from five different experiments
The signal intensities of Western blot bands were quantitated with AIDA software, and values were normalized against the quantity of pUL97 immunoprecipitated under control conditions (T1|T1, no cyclin T1 depletion); ND means not determined; tagged versions of pUL97 derived from HCMV-infected are underlined, and plasmid-transfected cells are in italics.
| Tagged versions of pUL97 for assembly | T1|T1 | T1|− | −|− | −| T1 |
|---|---|---|---|---|
| Derived from HCMV-infected cells | ||||
| pUL97–FLAG|pUL97–HA | 100% | 30% | 19% | 23% |
| pUL97–FLAG|pUL97–HA | 100% | 93% | 57% | 57% |
| pUL97–FLAG|pUL97–HA | 100% | 98% | 56% | 36% |
| Mean | 100 ± 0% | 74 ± 38% | 44 ± 22% | 39 ± 17% |
| Derived from plasmid-transfected cells | ||||
| pUL97–HA|pUL97–FLAG | 100% | 88% | 59% | 55% |
| Derived from infected and transfected cells | ||||
| pUL97–HA |pUL97–FLAG | 100% | 63% | 76% | ND |