Figure 7.

TabFH2 binds ATTR ex vivo seeds. Increasing amounts of TabFH2 were added to independent wells pretreated with ATTR seeds (treated with seeds) or buffer (treated with buffer), and unbound TabFH2 was detected by reverse-phase chromatography. TabFH2 samples before treatment were included in the analysis (untreated). a, representative HPLC elution profile for a TabFH2 concentration of 500 μm showing two peaks: TabH2 eluting at ∼17 ml and TabF2 eluting at ∼21 ml. b, analysis of TabFH2 recovery at all concentrations, measured as peak area integrated from HPLC elution profiles. The graph shows that the recovery of both TabF2 and TabH2 when wells were pretreated with ATTR seeds was significantly lower than when pretreated with buffer.