Figure 1.

YY1 functions as a tumor suppressor in nasopharyngeal carcinoma. A, Western blotting using antibodies against YY1 and FLAG tag to confirm exogenous YY1 protein levels. GAPDH served as an internal control (Ctrl). B, CCK-8 assays of 5-8F and HNE2 stably overexpressing YY1 or negative control cells. C, colony-forming assays (upper panel) and quantification of colony 800/well (lower panel). D, cell-cycle analysis by flow cytometry. E, flow-cytometry analysis of cell apoptosis via annexin V-PE and 7-AAD double staining. Error bars represent the mean ± S.D. *, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, no significance. All experiments were performed in triplicate.