Figure 2.

Depletion of YY1 by siRNA promotes cell proliferation and inhibits apoptosis in NPC cell lines. A, 5-8F or HNE2 cells were transfected with scramble siRNA (negative control) and YY1 siRNA pool, respectively, and Western blotting was used to analyze the silence efficiency of YY1, and GAPDH served as an internal control. B, CCK-8 assays of 5-8F and HNE2 with transfection of YY1 siRNAs or negative control. C, colony-forming assays and quantification of colony 800/well (lower panel). D, cell-cycle analysis by flow cytometry. E, flow-cytometry analysis of cell apoptosis via annexin V-PE and 7-AAD double staining. Error bars represent the mean ± S.D. *, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, no significance. All experiments were performed in triplicate.