Skip to main content
. 2019 Feb 4;294(15):6172–6187. doi: 10.1074/jbc.RA118.006281

Figure 4.

Figure 4.

YY1 negatively regulates c-Myc–mediated miR-141 transcription. A, dual-luciferase reporter assay shows the effect of YY1 overexpression on miR-141 promoter activity regulated by c-Myc in 5-8F and HNE2 cells. Data are normalized to Renilla activity (pRL-TK). B, qRT-PCR analysis shows the effect of YY1 overexpression on miR-141 levels regulated by c-Myc in 5-8F and HNE2 cells. U6 served as an internal control. C and D, dual-luciferase reporter assay and qRT-PCR analysis show miR-141 promoter activity and pri-miR-141, pre-miR-141, and mature miR-141 levels in YY1-overexpressing 5-8F (C) and HNE2 (D) cells. U6 served as an internal control. E, Western blotting of Dicer and Drosha protein levels in YY1-overexpressing 5-8F and HNE2 cells. GAPDH served as an internal control. F, Western blotting was performed to confirm the protein levels of exogenous YY1 and endogenous c-Myc by using antibodies against FLAG tag and c-Myc in 5-8F and HNE2 cells. GAPDH served as an internal control. G, dual-luciferase reporter assay shows the effect of YY1overexpression on miR-141 promoter activity in c-Myc–knockdown 5-8F and HNE2 cells. Data are normalized to Renilla activity (pRL-TK). H, qRT-PCR analysis shows the effect of YY1 overexpression on miR-141 levels in c-Myc–knockdown 5-8F and HNE2 cells. U6 served as an internal control. Error bars represent the mean ± S.D. **, p < 0.01; ***, p < 0.001. All experiments were performed in triplicate.