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Restoring miR-141 levels reverses the effect of YY1 on cell proliferation and colony formation in NPC cells. A, Western blotting using antibodies against FLAG and the apoptosis marker c-PARP. GAPDH served as an internal control. B, CCK-8 assays of 5-8F and HNE2 stably overexpressing YY1 or negative control (Ctrl). C, colony-forming assays and quantification of colony 800/well. Error bars represent the mean ± S.D. ***, p < 0.001. All experiments were performed in triplicate.
