Figure 7.

YY1 inhibits tumor growth by down-regulating miR-141 expression in vivo. A, representative images (top panel) and tumor images (bottom panel) of the 5-8F xenograft model in nude mice. B, tumor weight quantification (n = 5). C, tumor growth curve (n = 5). D, Western blotting using anti-FLAG antibodies confirmed YY1 protein levels in xenograft tumor tissues. GAPDH served as an internal control. E, qRT-PCR assay confirming the expression of mature miR-141 in xenograft tumor tissues. U6 served as an internal control. F, representative IHC images of tumor samples that were stained with hematoxylin and eosin (H&E) and Ki67 (left). The percentages of Ki67-positive cells were measured (right). Three tumors were analyzed per group. Original magnification, ×200; scale bars, 50 μm. Error bars represent the mean ± S.E. **, p < 0.01; ***, p < 0.001.