YY1 inhibits tumor growth by repressing miR-141/PTEN/AKT signaling.
A, relative protein expression levels in miR-141 mimic-transfected 5-8F and HNE2 stable cell lines were analyzed by Western blotting. GAPDH served as an internal control. B, IHC (DAB staining) for PTEN, p-AKT, p21, CCND1, and c-PARP in the 5-8F xenograft model. Three tumors were analyzed per group. Original magnification, ×200; scale bars, 50 μm. Error bars represent the mean ± S.D. Control: vector + miR-NC; YY1: YY1 + miR-NC. Error bars represent the mean ± S.D. **, p < 0.01; ***, p < 0.001.