Figure 1.
Myc-tagged candidates are expressed at similar levels and traffic to the cell surface. A, immunoblots of RIPA-soluble cell lysates following cell surface biotinylation and immunoprecipitation using anti-Myc-agarose beads. Candidate receptors were immunoblotted with an anti-Myc antibody (left) and fluorescently conjugated streptavidin (right). Empty, empty vector control. B, images of COS-7 cells transfected with the indicated candidate receptor and stained with anti-Myc antibody. Scale bar, 200 μm. C, quantification of B. All samples are normalized to hPrPC Myc signal. n = 32 experiments for hPrPC and 8–14 for all others. Individual data points indicate different experiments. Error bars, S.D.