Figure 3.
PrPC and NgR1 preferentially bind neurotoxic Aβo. A, SEC trace of 50 μm BAβo showing absorbance at 280 nm. Alternate vertical columns indicate fractions collected for analysis. Yellow fraction, HMW Aβ. Elution times of various standards (kDa) are indicated by arrows. V0, void volume as determined by blue dextran, 2,000 kDa. B, detection and quantification of the effect of the preparative method on the distribution of BAβ in different SEC fractions by dot blot analysis. C, quantification of expression-normalized Aβ signal from COS-7 cells transfected with the indicated candidate receptor and incubated with 1 μm monomeric biotin Aβ at 4 °C. Values are normalized to oligomeric biotin Aβ binding to hPrPC. One-sided t test comparing with an expected value of 100. n = 3–5 experiments. D, binding of receptors to monomeric Aβ compared with Aβo from Fig. 2B. Shown are multiple t tests with Holm–Sidak correction for multiple comparisons. Individual data points indicate different experiments. Error bars, S.D. n.s., not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.