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. 2019 Apr 5;10:213. doi: 10.3389/fendo.2019.00213

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Copyright © 2019 Liu, Kinoshita, Adolfi and Schartl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of mc4r knockout on medaka growth and puberty. (A) No differences in puberty onset timing in the three trials (A1, A2, A3), but some differences in body length at puberty between WT and KO medaka females and males in three replicates (A4, A5, A6). Data are presented as mean ± SD. Statistical significance after two-way ANOVA is indicated as asterisks (*). (B) The standard length of WT and KO during growth in the three experiments (B1, B2, B3). Data are presented as mean±SD. Statistical significance after multiple t-test is indicated as asterisks (*). For all statistics: ns: not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.