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. 2017 Oct 6;67(1):58–70. doi: 10.2337/db16-1587

Figure 4.

Figure 4

Overexpression of Cdk5rap1 or Cdkal1 increases the number of β-cells. A: Scheme of constructs used for overexpressing cdk5rap1 or cdkal1 under the control of tp1 promoter and expressing GFP in the heart (as an internal control of genomic integration). Relative mRNA levels of cdk5rap1 (B) or cdkal1 (C) normalized to the housekeeping gene actb1 in WT larvae and stable transgenic larvae overexpressing cdk5rap1 (B) or cdkal1 (C) at 6 dpf. **P ≤ 0.01; ***P ≤ 0.001. n = 5–8. D–I: Overexpressing Cdk5rap1 or Cdkal1 promoted β-cell neogenesis. Representative confocal images of the pancreas of Tg(ins:H2BGFP) larvae with or without the overexpression transgene, Tg(tp1:cdk5rap1) (D and E) or Tg(tp1:cdkal1) (G and H), at 6 dpf treated with LY from 3 to 6 dpf, displaying β-cells in green (arrowheads mark β-cells along the pancreatic tail). Dashed lines show the outline of the pancreas. Scale bar = 50 μm. F and I: Quantification of ins:H2BGFP+ cells in the pancreatic tail per larva at 6 dpf. *P ≤ 0.05; **P ≤ 0.01. n = 19–20.