Figure 7.

Roscovitine promotes β-cell formation in adult mouse pancreas after ductal ligation. A and B: Representative images of the duct-ligated part of pancreas (PDL tail) after treatment with PBS or roscovitine via osmotic pumps for 2 weeks, displaying INS⁺ cells in green, Ki67⁺ cells in red, CK19⁺ cells in yellow, and DAPI in blue. Insets show magnified representative INS⁺ cells in the periductal area. Arrowheads and arrows indicate INS⁺ cells in the periductal area and proliferating INS⁺ cells, respectively. Scale bar of main figures = 100 μm. Scale bar of insets = 25 μm. Quantification of INS⁺ cells in the periductal area (C), percentage of Ki67⁺ INS⁺ cells (D), and percentage of Ki67⁺ CK19⁺ cells in the PDL tail (E). P values from cohort 1, cohort 2, and combined are shown; n = 9–14.