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. 2019 Apr 8;10:207. doi: 10.3389/fendo.2019.00207

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Copyright © 2019 Liu, Fu, Li, Liang, Li, Li, Sui, Dionigi and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing FOXD2-AS1 downregulates TERT. (A) Western blot analysis of TERT expression in the indicated thyroid cancer cells. α-Tubulin was detected as a loading control. (B) Real-time PCR analysis of TERT expression in the indicated thyroid cancer cells thyroid cancer cells. Transcript levels were normalized by GADPH expression. *P < 0.05. (C) Biotinylated FOXD2-AS1 was incubated with total extracts of thyroid cancer cells, targeted with streptavidin beads and the LSD1 or TERT proteins were analyzed by Western blot. LSD1 binding to FOXD2-AS1 was served as a positive control. (D) Half-life analysis of the TERT protein. Cells were treated with 20 μM cycloheximide (CHX) in the indicated times and then analyzed by Western blotting.