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. 2019 Apr 8;10:207. doi: 10.3389/fendo.2019.00207

Figure 6.

Figure 6

FOXD2-AS1 functions as a ceRNA to sponge miR-7-5p to regulate TERT expression. (A) Predicted recognition sites of miR-7-5p on FOXD2-AS1. (B) Real-time PCR analysis of miR-7-5p and miR-7-1-3p expression in the indicated thyroid cancer cells. Transcript levels were normalized by U6 expression. Error bars represent the mean ± s.d. of three independent experiments. *P < 0.05. (C,D) The interaction between wild-type FOXD2-AS1 and miR-7-5p or miR-7-1-3p in thyroid cancer cells was investigated by RNA immunoprecipitation assay. Real-time PCR was performed to examine the expression of miR-7-5p and miR-7-1-3p. *P < 0.05. (E,F) The interaction between the wild-type or mutant FOXD2-AS1 and miR-7-5p or miR-7-1-3p in thyroid cancer cells was investigated by RNA immunoprecipitation assay. Real-time PCR was performed to examine the expression of miR-7-5p and miR-7-1-3p. *P < 0.05. (G) The predicted miR-7-5p target sequence in 3'UTRs of TERT. (H) Western blotting of TERT expression in the indicated cells. α-Tubulin served as the loading control. (I) Inhibition of miR-7-5p reversed the inhibitory effects of silencing FOXD2-AS1 on sphere formation ability of thyroid cancer cells. *P < 0.05. (J) Inhibition of miR-7-5p reversed the inhibitory effects of silencing FOXD2-AS1 on the fraction of side population in thyroid cancer cells. *P < 0.05. (K) Inhibition of miR-7-5p reversed the inhibitory effects of silencing FOXD2-AS1 on the fraction of CD133+ in thyroid cancer cells. *P < 0.05.