Table 1. List of applied DNA extraction methods, with the costs per sample, preparation and lysis time.
| DNA extraction method | Company | Method | Cost per sample1 (€) | Not included2 | Preparation time3 (min) | Lysis time (h) | Source |
|---|---|---|---|---|---|---|---|
| NucleoSpin Tissue XS (NST) | Macherey-Nagel | Silica membrane with spin columns | 3.12 | Ethanol 2 × 8 tubes RNase | 40–45 | 3–4 | http://www.mn-net.com/Products/DNAandRNAPurification/DNAfromtissueandcells/NucleoSpinTissueXS/tabid/10645/language/en-US/Default.aspx |
| QIAamp DNA Mini kit (QIA) | Qiagen | Silica membrane with spin columns | 3.46 | Ethanol 2 × 8 tubes RNase | 50 | 3 | https://www.qiagen.com/at/shop/sample-technologies/dna/genomic-dna/qiaamp-dna-mini-kit/#orderinginformation |
| PeqGOLD Tissue DNA Mini kit (PEQ) | peqlab | Silica membrane with columns | 1.414 | Ethanol 3 × 8 tubes | 40–45 | 3 | https://de.vwr.com/store/product/16763411/tissue-dna-mini-kits-peqgold |
| Wizard Genomic DNA kit (WIP) | Promega | Protein precipitation method | 1.685 | 70% Ethanol, Isopropanol 3 × 8 tubes | 55 | 3 | https://www.promega.com/products/dna-purification-quantitation/genomic-dna-purification/wizard-genomic-dna-purification-kit/ |
| CTAB | SIGMA | Cetyltrimethyl ammonium bromide, 2 phase-chloroform precipitation method | 1.006 0.6–0.87 | NaCl, EDTA, TrisHCl, Proteinase K, β-mercaptoethanol, Chloroform, Isopropanol, Ethanol, TE-buffer 3 × 8 tubes RNase | 1358 | 3 | http://www.sigmaaldrich.com/catalog/product/sigma/h6269?lang=de®ion=AT |
| E.Z.N.A. Insect DNA kit (EZNA) | Omega Bio-tek, Inc. | Cetyltrimethyl ammonium bromide based method with a silica membrane with spin columns | 1.72 | Ethanol, Isopropanol, Chloroform, Isoamyl alcohol 3 × 8 tubes | 80 | 4 | https://at.vwr.com/store/product/10925134/e-z-n-a-insect-dna-kit |
| CHELEX (CH) | Bio-Rad | Chelating ion exchange method | 0.02 | Water 8 tubes RNase (Proteinase K) | 1.5 | 0.20 | http://www.bio-rad.com/de-at/sku/1421253-chelex-100-resin |
Notes:
list price for 50 samples, not included substances (for the commercially available kits) and disposables (additional tubes, pipette tips) were excluded; for CTAB all substances listed in table were included in calculation; Chelex was calculated for 50 g.
for all methods manual pipettors with disposable tips, a centrifuge, a vortex mixer and a thermal heating-block are required; for CTAB and the EZNA a fume hood is required; for the WIP a vacuum centrifuge was used.
for eight samples excl. lysis and RNase incubation steps, incl. waiting steps (centrifugation, cooling, etc.).
calculated with a 50% reduced volume and the price of 25 columns was added.
calculated with a 50% reduced volume.
Chen et al. (2010) (including microcentrifuge tubes and pipette tips) and Kranzfelder, Ekrem & Stur (2016).
Isopropanol precipitation step (overnight) and time spent for buffer preparation was excluded.