Table 2. Concentration measurements (ng/μL) of DNA extracts by fluorometry (QuantiFluor), obtained from three oribatid mite species.
Extraction method | Tectocepheus sp. | Paraleius leontonychus | Hermannia sp. | |||
---|---|---|---|---|---|---|
NST (25 µl) | 0.003 | 0.002 | 0.147 | 0.329* | 0.020 | 0.015 |
QIA (50 µl) | 0.053 | 0.016* | 0.115 | 0.135* | 0.969 | 0.770 |
PEQ (50 µl) | 0.005 | 0.0073 | 0.014 | 0.048 | 0.096 | 0.039 |
WIP (30 µl) | x1 | x1,* | x1 | 0.002* | 0.176 | 0.015 |
CTAB (25 µl) | 0.041 | 0.031 | 0.102 | 0.039* | 0.006 | 0.021 |
EZNA (75 µl) | 0.021 | 0.015 | 0.063 | 0.071* | 0.696 | – |
CH1 (50 µl) | 0.054 | 0.081* | 0.115 | 0.095* | 0.506 | 1.460 |
CH2 (50 µl) | 0.024 | 0.014 | 0.040 | 0.048* | 0.217 | 0.285 |
CH3 (50 µl) | 0.016 | 0.023 | 0.027 | 0.053* | 0.288 | 0.332 |
Notes:
Same samples per species are indicated by same shades (white or gray) as found in Table S2. Values are given for single individuals (two per species). For the EZNA only one Hermannia individual was investigated. Values based on one measurement (control measurements revealed no deviating values). Elution volumes are given in parenthesis.
x = value lower than blank.
no PCR product.
specimens preserved in propylene glycol.