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. 2019 Apr 12;7:e6753. doi: 10.7717/peerj.6753

Table 2. Concentration measurements (ng/μL) of DNA extracts by fluorometry (QuantiFluor), obtained from three oribatid mite species.

Extraction method Tectocepheus sp. Paraleius leontonychus Hermannia sp.
NST (25 µl) 0.003 0.002 0.147 0.329* 0.020 0.015
QIA (50 µl) 0.053 0.016* 0.115 0.135* 0.969 0.770
PEQ (50 µl) 0.005 0.0073 0.014 0.048 0.096 0.039
WIP (30 µl) x1 x1,* x1 0.002* 0.176 0.015
CTAB (25 µl) 0.041 0.031 0.102 0.039* 0.006 0.021
EZNA (75 µl) 0.021 0.015 0.063 0.071* 0.696
CH1 (50 µl) 0.054 0.081* 0.115 0.095* 0.506 1.460
CH2 (50 µl) 0.024 0.014 0.040 0.048* 0.217 0.285
CH3 (50 µl) 0.016 0.023 0.027 0.053* 0.288 0.332

Notes:

Same samples per species are indicated by same shades (white or gray) as found in Table S2. Values are given for single individuals (two per species). For the EZNA only one Hermannia individual was investigated. Values based on one measurement (control measurements revealed no deviating values). Elution volumes are given in parenthesis.

x = value lower than blank.

1

no PCR product.

*

specimens preserved in propylene glycol.