Table 3. Performance of 12 different DNA extraction protocols.
PCR and sequencing success | Quality of DNA | Quantity of DNA1 | Purity of DNA | Costs | Handling time | User friendliness | Plastic waste | Hazardous waste | |||
---|---|---|---|---|---|---|---|---|---|---|---|
NST | + | + | ∼ | − | − | + | + | ∼ | + | ||
QIA | + | + | ++ | − | − | + | + | ∼ | + | ||
PEQ | ∼ | − | ∼ | * | ∼ | + | + | − | + | ||
WIP | − | − | ∼ | − | ∼ | + | ∼ | − | + | ||
EZNA | + | + | + | − | ∼ | − | − | − | − | ||
CTAB | + | − | ∼ | − | + | − | − | − | − | ||
CH1 | + | − | ++ | + | ++ | ++ | ++ | ++ | + | ||
CH2 | + | − | + | ∼ | ++ | ++ | ++ | + | + | ||
CH3 | + | ∼ | + | − | ++ | ++ | ++ | + | + | ||
CH4–CH6 | + | +a | −b | +c | + | * | ++ | ++ | ++ | + | + |
Notes:
++ = very good, + = good, ∼ = moderate, − = negative; these symbols are based on author’s personal opinion and experiences.
based on measurements with the Quantus Fluorometer.
CH4.
CH5.
CH6.
not measured.