Figure 2.

G0S2 promotes TG synthesis in an ATGL-independent way. A, B) Primary hepatocytes infected with 2 × 10⁷ of Ad-Null or G0S2-expressing adenovirus (Ad-G0S2) were treated with BSA-complexed oleate (0.3 mM unlabeled plus 1 µCi/ml [9,10-³H]oleate and 0.3% BSA) for 6 h. C) Immunoblotting (IB) of G0S2 and ATGL in cells was performed. D, E) Lipid synthesis was assessed by the rate of incorporation of [³H] oleate into TG (D) or DG (E). The data are representative of 3 independent experiments. Eight-week-old C57BL/6j female mice were injected with control (Ctrl KD) or G0S2-specific siRNA (G0S2 KD). Three days later, mice were unfed for 6 h. Then TG and DG species were profiled as described in Materials and Methods (n = 6). KD, knockdown; KO, knockout. *P < 0.05, **P < 0.01, ***P < 0.001.