Figure 3.

G0S2 possesses a LPAAT activity. A) Outline of the glycerol phosphate pathway for TG synthesis in liver. B, C) Evaluation of the GPAT or LPAAT activity of G0S2. C-terminal Flag-tagged G0S2, GPAT, or LPAAT was expressed using a yeast cell-free lysate and then immunoprecipitated by Flag agarose beads followed by the assay of GPAT (B) or LPAAT activity under standard conditions (C). D) The LPAAT activity was measured by using 10 µM [¹⁴C]oleoyl-CoA (oleoyl CoA*) in the presence or absence of 100 µM LPA (left panel) or by using 20 µM ([3H]) LPA (LPA*) in the presence or absence of 100 µM oleoyl-CoA (right panel). The data are representative of 3 independent experiments. ***P < 0.001 vs. vector alone.