Figure 6.

TM40DPS cells expressing mutant maspin lacking the CLBD translocate to mitochondria and display a low apoptosis efficiency. A) A plot comparing maspin translocated to mitochondria in TM40D^Mp (Mp^WT) and TM40D^PS cells (containing the corresponding sequence from PAI-1 swapped with the CLBD in maspin) after STS treatment; COX IV was used as a mitochondrial loading control. B) A plot comparing caspase 3 activity [optical density at 405 nm (OD405)] in TM40D^Mp and TM40D^PS cell lysates in the absence and presence of STS using a Colorimetric Assay Kit. C) A plot comparing cytochrome c (Cyt C) released into the cytosol was determined in TM40D^Mp and TM40D^PS cells after apoptosis induction. GAPDH was as used as a cytosolic loading control.