Figure 2.
Phenotypic profile of T cells harvested from skin explant cultures after 4 wk. Representative flow cytometry dot plots (left) and quantification statistics (right; n = 5–8) of the indicated surface markers on cells cultured under Th9-PC or SC (SSC/CD3, A; CD45RO/CD45RA, B; CD8/DC4, C, IL-9/CD3, D; CCR4/CLA, E; CD69/CD103, F). Cells (D) were αCD3 and αCD28 bead–stimulated and CSC-restimulated 3 d. Quadrants were set according to isotype-matched control staining. Ns, not significant. Means ± sem are shown, and data were analyzed using Wilcoxon’s rank-sum test. *P ≤ 0.05.