Figure 3.
Culture conditions alter CD69 and CD103 expression on skin T cells. A, B) Percentage of CD69 and CD103 cells among skin T cells (FI) or harvested after 4 wk from skin explant cultures under indicated conditions. Paired data are means ± sem and were analyzed using repeated measures with 1-way ANOVA (Holm-Sidak post hoc). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. Only significant differences are indicated. C) T cells from 4-wk cultures were reseeded (d 0; 105 T cells/well) and stimulated as well as restimulated according to the scheme. Percentage of IL-9+ T cells upon reseeding T cells under indicated conditions. Before each analysis, cells were αCD3 and αCD28 bead–stimulated and CSC-restimulated 3 d at the indicated time points (n = 3). Data are means ± sem.