Figure 4.

The effect of maternal obesity on absolute concentrations of unlabeled FAs and on the uptake and incorporation of U[¹³C]-FAs in PHT cells. A) Absolute concentrations of unlabeled SFAs (PA), MUFAs (OA), and essential FAs (n-6 LCPUFA, LA, and n-3 LCPUFA, DHA) in PHT cells isolated from obese mothers (gray bars) compared with trophoblasts isolated from normal BMI mothers (Ctr; black bars) after incubation with U[¹³C]-FA mixture for 2 and 30 h. B) Uptake of U[¹³C]-FAs in PHT cells isolated from obese and normal BMI mothers after incubation with U[¹³C]-FA mixture for 2 and 30 h. Results are presented for the total cellular lipid pool and expressed as nanomoles of FA per microgram of protein for each labeled FA studied (n = 7 in each group, means + sem). *P < 0.05 (Mann-Whitney U test).