Figure 3.

Effects of simulated microgravity on cell cycle and apoptosis of HSPCs. A) Distribution of each phase of the cell cycle was evaluated in HSPCs after 12 d of culture under normal and simulated microgravity conditions. %CV (coefficient of variation) indicates the numerical dispersion degree. Total B.A.D (Background, Aggregates and Debris) indicates the amount of debris and aggregates found within the region between the first G₁ and the last G₂ peak. B) Ratio of G₁ and S-phase murine HSPCs after 12 d of culture under normal and simulated microgravity conditions. C) Expression of propidium iodide (PI) in cultured HSPCs under simulated microgravity for 12 d. µ-g, simulated microgravity; 1-g, normal gravity. **P < 0.01.