Figure 2.

Neuroprotective effect of PMSCs by indirect coculture. SH-SY5Y cells were subjected to staurosporine treatment and cocultured with PMSCs seeded onto hanging cell culture inserts. A–D) Calcein AM staining and Wimasis image analysis in the absence (A, C) and presence (B, D) of PMSCs show increased neurite outgrowth in the presence of PMSCs. E–H) WimNeuron image analysis showed a significant fold increase in total branching points (E), circuitry length (px) (F), and total segments (px) (G), and a significant increase in cell number (H) in the presence of PMSCs compared with controls (n = 4 donor cell banks and repeated 3 times). Scale bars, 200 µm.