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. 2019 Feb 28;33(5):6011–6022. doi: 10.1096/fj.201801993R

Figure 1.

Figure 1

Retrograde perfusion of the submandibular salivary gland with tcMCMV resulted in the expression of transcription factors associated with Th1/Th2/Th17 cytokines after a lethal challenge with MCMV. BALB/cByJ mice were inoculated with saline (mock) or 105 PFU/mouse tcMCMV via retrograde perfusion of the salivary gland. On d 28 after inoculation, saline mice were challenged intraperitoneally with 2 × 104 PFU/mouse MCMV, and tcMCMV mice were challenged intraperitoneally with 5 × 104 PFU/mouse MCMV. RNA was prepared from the PGLNs and the lymphocytes obtained from the whole salivary glands after removal of the associated lymph nodes in saline control (mock) (−/−), primed (+/−), or postchallenge (−/+ or +/+) mice on d 14 and analyzed for expression of the indicated transcription factors by qRT-PCR. A) Salivary glands. B) PGLNs. Data are the means ± sd of 5 mice/group. *P < 0.05, **P < 0.01, ***P < 0.001.