Figure 6.

Retrograde perfusion of the submandibular salivary gland with replication-deficient recombinant adenovirus expressing MCMV genes resulted in the expression of transcription factors associated with T_h1/T_h2/T_h17 responses. BALB/cByJ mice were inoculated (d 0) and boosted on d 30 with either saline, 10⁶ PFU/mouse of FG140, or a combination of Ad-gpB, -gpH, and -IE1 (10⁶ PFU/mouse of each recombinant virus, Ad-Combo) via retrograde perfusion of the submandibular salivary gland. On d 30 after boost, mice inoculated with saline were challenged intraperitoneally with saline and mice inoculated with FG140 or Ad-Combo were challenged intraperitoneally with 5 × 10⁴ PFU/mouse MCMV. RNA was prepared from the whole salivary glands after removal of the associated lymph nodes and from the PGLNs and were analyzed for expression of the indicated transcription factors by qRT-PCR. A) Salivary gland RNA was analyzed from mice inoculated (d 0) and boosted on d 30 (prechallenge) with saline (mock, open bars), 10⁶ PFU/mouse of FG140 (negative control, gray bars), or with Ad-Combo (solid bars) for expression of T_h-related transcription factors. B) RNA from the salivary glands and PGLNs at 2 wk postchallenge was analyzed from mice inoculated and boosted (d 0, d 30) with Ad-Combo and challenged with 5 × 10⁴ PFU/mouse MCMV (d 72) (solid bars) for expression of T_h-related transcription factors. Data are the means ± sd of ≤5 mice/group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.