Figure 7.

Retrograde perfusion of the submandibular salivary gland with replication-deficient recombinant adenovirus expressing MCMV genes resulted in the expression of cytokines associated with T_h1/T_h2/T_h17 responses. BALB/cByJ mice were inoculated (d 0) and boosted on d 30 with either saline, 10⁶ PFU/mouse of FG140, or a combination of Ad-gpB, -gpH, and -IE1 (10⁶ PFU/mouse of each recombinant virus, Ad-Combo) via retrograde perfusion of the submandibular salivary gland. On d 30 after boost, mice inoculated with saline were challenged intraperitoneally with saline, and mice inoculated with FG140 or Ad-Combo were challenged intraperitoneally with 5 × 10⁴ PFU/mouse MCMV. A) RNA was prepared from the whole salivary glands after removal of the associated lymph nodes and was analyzed for T_h-related cytokine expression at d 30 after primary inoculation and boost (prechallenge) and at 2 wk post-boost/challenge (d 74). Saline (open bars), FG140 (negative control, gray bars), and Ad-Combo (solid bars). Data were not available for FG140-inoculated mice after lethal challenge (d 74) due to death. B) Saliva samples were collected from mice inoculated with saline (control, open bars), from FG140 (negative control, gray bars), from Ad-Combo at d 30 after primary inoculation and boost (prechallenge, hashed bars), and from Ad-Combo mice challenged intraperitoneally with MCMV (d 74 postchallenge, solid bars) and were analyzed via a T_h1/T_h2/T_h17 cytokine bead array assay. Saliva was expressed as total picograms recovered to normalize for differences in the volume of saliva collected from individual animals. Data are the mean ± sd of ≤5 mice/group. *P < 0.05, **P < 0.01, ****P < 0.0001.