TABLE 1.
Th1/Th2/Th17 related primers used for qRT-PCR
| Primer sequence, 5−3′ |
||
|---|---|---|
| Gene | Forward | Reverse |
| T-bet | AGCAAGGACGGCGAATGTT | GGGTGGACATATAAGCGGTTC |
| GATA3 | CTCGGCCATTCGTACATGGAA | GGATACCTCTGCACCGTAGC |
| Foxp3 | CCCATCCCCAGGAGTCTTG | ACCATGACTAGGGGCACTGTA |
| ROR-γt | ACCTCCACTGCCAGCTGTGTGCTGTC | TCATTTCTGCACTTCTGCATGTAGACTGTCCC |
| IL-2 | TGAGCAGGATGGAGAATTACAGG | GTCCAAGTTCATCTTCTAGGCAC |
| IL-6 | GAGGATACCACTCCCAACAGACC | AGTGCATCATCGTTGTTCATACA |
| IL-10 | CCCTTTGCTATGGTGTCCTT | TGGTTTCTCTTCCCAAGACC |
| IL-12b | TGGTTTGCCATCGTTTTGCTG | ACAGGTGAGGTTCACTGTTTCT |
| IFN-γ | GCGGCTGACTGAACTCAGATTGTAG | TCACAGTTTTCAGCTGTATAGGG |
| TGF-β1 | CTCCCGTGGCTTCTAGTGC | GCCTTAGTTTGGACAGGATCTG |
| TNF-α | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
| GAPDH | CATGGCCTTCCGTGTTCCTA | GCGGCACGTCAGATCCA |