TABLE 1.
Transcription levels of methionine metabolism genes in the cochlea of E18.5 mice
| Symbol | Gene name | Expression level |
|---|---|---|
| Ahcy | S-adenosylhomocysteine hydrolase | 8.64 ± 0.02 |
| Ahcy1 | S-adenosylhomocysteine hydrolase-like 1 | 7.98 ± 0.05 |
| Amd1 | S-adenosylmethionine decarboxylase 1 | 8.77 ± 0.01 |
| Apip | Apoptotic peptidase activating factor 1 (APAF1) interacting protein | 7.22 ± 0.12 |
| Bhmt | Betaine-homocysteine methyltransferase | 2.74 ± 0.10 |
| Bhmt2 | Betaine-homocysteine methyltransferase 2 | 1.92 ± 0.10 |
| Carm1 | Coactivator-associated arginine methyltransferase 1 | 8.44 ± 0.07 |
| Cbs | Cystathionine β-synthase | 2.10 ± 0.29 |
| Cdo1 | Cysteine dioxygenase 1, cytosolic | 8.26 ± 0.05 |
| Cth | Cystathionase (cystathionine γ-lyase) | 4.93 ± 0.04 |
| Dnmt1 | DNA methyltransferase (cytosine-5) 1 | 8.42 ± 0.02 |
| Dnmt3a | DNA methyltransferase 3A | 8.21 ± 0.04 |
| Dnmt3b | DNA methyltransferase 3B | 4.96 ± 0.10 |
| Gclc | Glutamate-cysteine ligase, catalytic subunit | 6.95 ± 0.03 |
| Gclm | Glutamate-cysteine ligase, modifier subunit | 7.22 ± 0.03 |
| Got1 | Glutamate oxaloacetate transaminase 1, soluble | 7.15 ± 0.04 |
| Got2 | Glutamate oxaloacetate transaminase 2, mitochondrial | 9.92 ± 0.03 |
| Gnmt | Glycine N-methyltransferase | 0.69 ± 0.29 |
| Mat1a | Methionine adenosyltransferase I, α subunit | 0.00 ± 0.33 |
| Mat2a | Methionine adenosyltransferase II, α subunit | 8.83 ± 0.08 |
| Mat2b | Methionine adenosyltransferase II, β subunit | 7.64 ± 0.03 |
| Mcee | Methylmalonyl coenzyme A epimerase | 7.52 ± 0.02 |
| Msrb2 | Methionine sulfoxide reductase B2 | 6.26 ± 0.11 |
| Mtap | Methylthioadenosine phosphorylase | 6.80 ± 0.07 |
| Mtfmt | Mitochondrial methionyl-tRNA formyltransferase | 6.73 ± 0.08 |
| Mthfr | 5,10-Methylenetetrahydrofolate reductase | 7.99 ± 0.03 |
| Mtrr | 5-Methyltetrahydrofolate-homocysteine methyltransferase reductase | 6.05 ± 0.10 |
| Mut | Methylmalonyl-Coenzyme A mutase | 7.54 ± 0.03 |
| Pcca | Propionyl Coenzyme A carboxylase, α polypeptide | 8.00 ± 0.02 |
| Pccb | Propionyl Coenzyme A carboxylase, β polypeptide | 7.91 ± 0.12 |
| Pemt | Phosphatidyl ethanolamine methyl transferase | 4.08 ± 0.08 |
| Rnmt | RNA (guanine-7-) methyltransferase | 7.24 ± 0.04 |
| Shmt1 | Serine hydroxymethyl transferase 1 (soluble) | 6.37 ± 0.04 |
| Sms | Spermine synthase | 5.12 ± 0.06 |
| Srm | Spermidine synthase | 9.12 ± 0.02 |
|
Suox |
Sulfite oxidase | 6.33 ± 0.07 |
| Tat | Tyrosine aminotransferase | 2.58 ± 0.11 |
Gene expression was determined using the whole-transcriptome GeneChip Mouse Genome 430A 2.0 Array. RNA was isolated from 3 independent cochleae pools from C57BL/6J E18.5 mouse embryos (n = 6). Gene expression data of methionine cycle and related metabolic pathways were analyzed by puma Bioconductor package to obtain an expression level for the different probe sets on each array. Previously collected expression data from the different replicates were combined with the Probability of Positive Log Ratio (PPLR) method to give a single expression level (mean ± sd), as it is included in the table.