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. 2019 Feb 25;33(5):6632–6642. doi: 10.1096/fj.201802373R

Figure 5.

Figure 5

The SNP rs7940681 in the human ANO1 promoter region disrupts repression of ANO1 transcription by Glis. A) Schematic of the ANO1 promoter showing the localization and the sequence changes in the presence of the rs7940681 SNP. B) Expression of the human ANO1 promoter-luciferase chimeric construct modified with the rs7940681 A-to-G SNP transfected in the HEK293 cell line. Promoter activity was calculated by fold increase over the luciferase activity of the empty vector (n = 6). *P < 0.05, 1-way ANOVA with Dunn’s multiple comparisons test. C) Schematic of the plasmid containing the control promoter sequence (CTRL) and the plasmid containing the SNP sequence (SNP) used for transient ChIP (TChIP) experiment. D) Transient ChIP–quantitative PCR experiment to test whether rs7940681 directly inhibits Gli binding to the ANO1 promoter. The data are indicated as relative enrichments over input (n = 4). *P < 0.05, 2-way ANOVA with Sidak’s multiple comparison test.