Figure 13.

The effect of smyd1a and smyd1b deficiency on myosin expression in early-stage zebrafish embryos. Zebrafish embryos from WT or smyd1a^mb5;smyd1b^sa15678 mutant were fixed at 14 hpf. A–F) Slow MyHC 1 mRNA and protein expression were analyzed by whole-mount in situ hybridization (A, B) and antibody staining (D, E), respectively. The images represent dorsal view of the embryos. In situ hybridization comparing MyoD gene expression in WT control (A) or smyd1a^mb5; smyd1b^sa15678 mutant (B) embryos at 14 hpf. C) qRT-PCR analysis compared the levels of Smyhc and Fmyhc mRNA transcripts in WT control and smyd1a^mb5; smyd1b^sa15678 mutant embryos at 24 hpf. Immunostaining with anti-MHC (F59) shows the MyHC protein expression in WT control (D) or smyd1a^mb5; smyd1b^sa15678 mutant (E) embryos at 14 hpf. The muscle segments/somites are indicated by the boxes. F) Western blot analysis compared the levels of MyHC protein expression in smyd1a^mb5, smyd1a^mb5; smyd1b^sa15678 mutants and WT control embryos at 24 and 48 hpf. Scale bars: 150 µm (A); 25 µm (D).