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Knockout of smyd1b by CRISPR disrupted sarcomere organization in slow myofibers. The Smyd1b-sgRNA was coinjected with Cas9 protein into Myom3mn0067gt zebrafish embryos at 1–2 cell stages. Myom3-RFP expression (A, B) and anti-sMyHC (F59) immunostaining (C, D) show the respective M line and thick filament organization in slow fibers of control (A, C) and Smyd1b-sgRNA/Cas9–injected (B, D) embryos at 24 hpf. E, F) Merged images of A, C or B, D, respectively. Scale bars, 20 μm (A, B).
