Figure 6.

Smyd1a-sgRNA design and indel mutations generated by CRISPR in the zebrafish smyd1a gene. A) The Smyd1a-sgRNA was designed to target the exon 3 sequence in the smyd1a gene in zebrafish. The Smyd1a-sgRNA was coinjected with Cas9 mRNA into WT zebrafish embryos at 1–2 cell stages. Two mutant alleles (smyd1a^mb4 and smyd1a^mb5) were generated that contain a 7 or 10 bp deletion, respectively. B) The smyd1a^mb4 and smyd1a^mb5 mutant alleles contained a reading frame shift mutation at position 147 and 146 with respect to the ATG start codon, respectively, leading to premature termination and truncation of almost 2/3 of the Smyd1a protein containing the essential middle and C-terminal regions.