Figure 7.

Loss of smyd1a had no effect on myogenesis and fish growth. A–C) In situ hybridization comparing smyd1a mRNA expression in WT control (A), smyd1a^mb4 (B), and smyd1a^mb5 (C) mutant embryos at 48 hpf. D) qRT-PCR analysis compared the levels of smyd1a mRNA transcripts in WT control and smyd1a^mb5 mutant embryos at 24 and 48 hpf. *P < 0.0001. E–J) In situ hybridization comparing MyHC gene expression in slow (E–G) and fast (H–J) muscles in WT control (E, H) or smyd1a^mb4 (F, I) and smyd1a^mb5 (G, J) mutant embryos at 24 hpf. The Smyhc1 and Fmyhc probes are slow and fast muscle specific, respectively. K–M) Morphologic comparison of WT control (K), smyd1a^mb4 (L), and smyd1a^mb5mutant fish (M) at 90 d old. Scale bars: 150 μm (C); 120 μm (G, J); 700 μm (M).