TABLE 1.
Primers for sgRNA synthesis and PCR analyses
| Primer | Sequence, 5′–3′ |
|---|---|
| Smyd1a-Cas-P1 | TAGGACCTGAAGGAGCTCAAAG |
| Smyd1a-Cas-P2 | AAACCTTTGAGCTCCTTCAGGT |
| Smyd1b-sgRNA-F1 | GATCACTAATACGACTCACTATAGAATCAGCTGACGACTCTGGGTTTTAGAGCTAGAAAT |
| Cas9-P4 | AAAAGCACCGACTCGGTGCC |
| Smyd1a-F2 | AGTCTGGTTGCTCGTATCCTGTGGCG |
| Smyd1a-R2 | CTGCTGTCAAATTGTGTGCTGAAGC |
| Smyd1b-sg4/5-F1 | AGTCTGGCGGCCCGTATCCTGTGGC |
| Smyd1b-sg4/5-R1 | ACCACGCCGAGGATGTGCGAGACGC |
| Smyd1b-mut-F1 | CGTGTGAATGGGGCGTCATAGATGTG |
| Smyd1b-mut-R2 | GGCCAGCAGTCGTGGTTCACCAAAC |
| zfhsp40-P9-qPCR | ATCAGCACGCTGGACAACAGATCGC |
| Zfhsp40-P10-qPCR | CGGTCAGCATGATGTGCTCTCGTAC |
| zfhsp90-α1-P6-qPCR | AGCCAGACTTCGGTGAATCAA |
| zfhsp90-α1-P7-qPCR | TTCTCTCTGTTTCTCAATGTAAA |
| zfmyhz2-P4-qPCR | GCTCACCTACCAGACTGAGGA |
| zfmyhz2-P5-qPCR | ACTCAGCAATATCAGCACGCT |
| zfsmyhc1-P4 | GCTCACCTACCAGACTGAGGA |
| zfsmyhc1-P5-qPCR | CATCTTGTTGACCTGAGATTCA |
| zfef1a-P3-qPCR | CTTCAACGCTCAGGTCATCAT |
| zfef1a-P4-qPCR | ACAGCAAAGCGACCAAGAGGA |
| zfSmyd1b-Bopx-qPCR | AAAGCCTTCGCCATCCTGATGATCAC |
| zfSmyd1b-Bop4-qPCR | CGTTGACCTGAGCGTGTCTGTCACTGTGG |
| zfSmyd1a-P1-qPCR | AGCATGACCGTGGAGAAGACGGAC |
| zfSmyd1a-E2-R1-qPCR | GACACGTGCGGTCGCAGTAATGCGC |
The underlined sequences in Smyd1a-Cas-P1 and Smyd1b-sgRNA-F1 are the gene-specific target sequences within the respective sgRNAs. Mut, mutation.