TABLE 1.
Details of primary and secondary antibodies used in this study
Antibody | Source | Dilution | Catalog no. |
---|---|---|---|
Primary antibodies | |||
Goat anti-calretinin | Swant, Marly, Switzerland | 1:1000 | CG1 |
Chicken anti-GFAP | Abcam, Cambridge, MA, USA | 1:1000 | AB4674 |
Biotinylated anti-mouse HuC/D | Thermo Fisher Scientific | 1:200 | A21272 |
Rabbit anti–IL-1β | Abcam | 1:500 | AB9722 |
Sheep anti-nNOS | MilliporeSigma | 1:500 | AB1529 |
Secondary antibodies | |||
Alexa Fluor 350–conjugated streptavidin | Thermo Fisher Scientific | 1:200 | S-11249 |
Alexa Fluor 488 goat anti-chicken | Thermo Fisher Scientific | 1:200 | A-11039 |
Alexa Fluor 488 donkey anti-goat | Jackson ImmunoResearch Laboratories, West Grove, PA, USA | 1:200 | 705-545-003 |
Alexa Fluor 488 donkey anti-sheep | Jackson ImmunoResearch Laboratories | 1:200 | 713-545-003 |
Alexa Fluor 568 donkey anti-goat | Thermo Fisher Scientific | 1:200 | A-11057 |
Alexa Fluor 594 donkey anti-rabbit | Jackson ImmunoResearch Laboratories | 1:200 | 711-585-152 |
Alexa Fluor 594–conjugated streptavidin | Jackson ImmunoResearch Laboratories | 1:200 | 016-580-084 |