Table 1.
Preanalytical Variables Included in SPREC (7-element long SPREC), Version SPREC 2.0, Applied to Fluid Samples
| Type of sample | |
|---|---|
| Ascites fluid | ASC |
| Amniotic fluid | AMN |
| Bronchoalveolar lavage | BAL |
| Blood (whole) | BLD |
| Bone marrow aspirate | BMA |
| Breast milk | BMK |
| Buccal cells | BUC |
| Unficolled buffy coat, viable | BUF |
| Unficolled buffy coat, nonviable | BFF |
| Ficoll mononuclear cells, viable | CEL |
| Fresh cells from nonblood specimen type | CEN |
| Cells from nonblood specimen type(e.g., ascites, amniotic), viable | CLN |
| Cord blood | CRD |
| Cerebrospinal fluid | CSF |
| Dried whole blood (e.g., Guthrie cards) | DWB |
| Nasal washing | NAS |
| Ficoll mononuclear cells, nonviable | PEL |
| Cells from non blood specimen type (e.g., ascites, amniotic), nonviable | PEN |
| Pleural fluid | PFL |
| Plasma, single spun | PL1 |
| Plasma, double spun | PL2 |
| Red blood cells | RBC |
| Saliva | SAL |
| Semen | SEM |
| Serum | SER |
| Sputum | SPT |
| Stool | STL |
| Synovial fluid | SYN |
| Tears | TER |
| 24 h urine | U24 |
| Urine, random (“spot”) | URN |
| Urine, first morning | URM |
| Urine, timed | URT |
| Other | ZZZ |
| Type of primary container | |
|---|---|
| Acid citrate dextrose | ACD |
| Additives | ADD |
| Serum tube without clot activator | CAT |
| Citrate phosphate dextrose | CPD |
| Cell Preparation Tube® | CPT |
| EDTA and gel | EDG |
| Lithium heparin | HEP |
| Hirudin | HIR |
| Lithium heparin and gel | LHG |
| Oragene collection container or equivalent | ORG |
| PAXgene® blood RNA+ | PAX |
| Potassium EDTA | PED |
| Polyethylene tube sterile | PET |
| S8820 protease inhibitor tablets or equivalent | PI1 |
| Protease inhibitors | PIX |
| Polypropylene tube sterile | PPS |
| PAXgene® blood DNA | PXD |
| PAXgene® bone marrow RNA | PXR |
| Sodium citrate | SCI |
| Sodium EDTA | SED |
| Sodium heparin | SHP |
| Sodium fluoride/potassium oxalate | SPO |
| Serum separator tube with clot activator | SST |
| Tempus® tube | TEM |
| Trace elements tube | TRC |
| Unknown | XXX |
| Other | ZZZ |
| Pre-centrifugation (delay between collection and processing) | ||
|---|---|---|
| RT* | <2 h | A |
| 2°C–10°C | <2 h | B |
| RT | 2–4 h | C |
| 2°C–10°C | 2–4 h | D |
| RT | 4–8 h | E |
| 2°C–10°C | 4–8 h | F |
| RT | 8–12 h | G |
| 2°C–10°C | 8–12 h | H |
| RT | 12–24 h | I |
| 2°C–10°C | 12–24 h | J |
| RT | 24–48 h | K |
| 2°C–10°C | 24–48 h | L |
| RT | >48 h | M |
| 2°C–10°C | >48 h | N |
| 35°C–38°C | <2 h | O |
| Unknown | X | |
| Other | Z | |
| Centrifugation | ||
|---|---|---|
| RT 10–15 min | <3000 g no braking | A |
| RT 10–15 min | <3000 g with braking | B |
| 2°C–10 °C 10–15 min | <3000 g no braking | C |
| 2°C–10 °C 10–15 min | <3000 g with braking | D |
| RT 10–15 min | 3000–6000 g with braking | E |
| 2°C–10°C 10–15 min | 3000 g to 6000 g with braking | F |
| RT 10–15 min | 6000 g to 10000 g with braking | G |
| 2°C–10 °C 10–15 min | 6000 g to 10000 g with braking | H |
| RT 10–15 min | >10000 g with braking | I |
| 2°C–10 °C 10–15 min | >10000 g with braking | J |
| RT 30 min | <1000 g no braking | M |
| No centrifugation | N | |
| Unknown | X | |
| Other | Z |
| Second centrifugation | ||
|---|---|---|
| RT 10–15 min | <3000 g no braking | A |
| RT 10–15 min | <3000 g with braking | B |
| 2°C–10°C 10–15 min | <3000 g no braking | C |
| 2°C–10°C 10–15 min | <3000 g with braking | D |
| RT 10–15 min | 3000–6000 g with braking | E |
| 2°C–10°C 10–15 min | 3000–6000 g with braking | F |
| RT 10–15 min | 6000–10,000 g with braking | G |
| 2°C–10°C 10–15 min | 6000–10,000 g with braking | H |
| RT 10–15 min | >10,000 g with braking | I |
| 2°C–10°C 10–15 min | >10,000 g with braking | J |
| No centrifugation | N | |
| Unknown | X | |
| Other | Z |
| Post-centrifugation delay | |
|---|---|
| <1 h 2°C–10°C | A |
| <1 h RT | B |
| 1 to 2 h 2°C–10°C | C |
| 1 to 2 h RT | D |
| 2 to 8 h 2°C–10°C | E |
| 2 to 8 h RT | F |
| 8 to 24 h 2°C–10°C | G |
| 8 to 24 h RT | H |
| >24 h 2°C–10°C | I |
| >24 h RT | J |
| Not applicable | N |
| Unknown | X |
| Other | Z |
| Long-term storage | ||
|---|---|---|
| PP tube 0.5- to 2 mL** | (−85)°C–(−60)°C | A |
| PP tube 0.5- to 2 mL | (−35)°C–(−18)°C | B |
| PP tube 0.5- to 2 mL | <-135°C | V |
| Cryotube 1- to 2 mL | LN*** | C |
| Cryotube 1- to 2 mL | (−85)°C–(−60) °C | D |
| Cryotube 1- to 2-mL | Programmable freezing to <-135°C | E |
| Plastic cryo straw | LN*** | F |
| Straw | (−85)°C–(−60)°C | G |
| Straw | (−35)°C–(−18)°C | H |
| Straw | Programmable freezing to <-135°C | I |
| PP tube ≥5 mL | (−85)°C–(−60)°C | J |
| PP tube ≥5 mL | (−35)°C–(−18)°C | K |
| Microplate | (−85)°C–(−60)°C | L |
| Microplate | (−35)°C–(−18)°C | M |
| Cryotube 1- to 2 mL | LN*** after temporary (−85)°C–(−60)°C | N |
| Plastic cryo straw | LN*** after temporary (−85)°C–(−60)°C | O |
| Paraffin block | RT or 2°C–10°C | P |
| Bag | LN*** | Q |
| Dry technology medium | RT | R |
| PP tube 40- to 500 μL | (−85)°C–(−60)°C | S |
| PP tube 40- to 500 μL | (−35)°C–(−18)°C | T |
| PP tube 40- to 500 μL | <-135°C | W |
| Original primary container | (−35)°C–(−18)°C or (−85)°C–(−60)°C | Y |
| Unknown | X | |
| Other | Z |
New elements are screened. Codes in bold come from the Laboratory Data Management System (LDMS).
*
RT, room temperature: 18°C–28°C; **PP, polypropylene; ***LN, liquid nitrogen, referring to either vapor- or liquid-phase (this information being documented in the biobank's SOPs)
Volumes refer to container size.