HIV-1 antigen-positive immunoblots of CVL samples and corresponding Coomassie-stained SDS gels. (a) Of the 20 CVL samples analyzed for the presence of HIV-1 p24 at serial clinic visits, the WB data of six representative CVL samples are shown. Lane 3 of both Gel 1 and Gel 2 represents the same CVL sample. Gel 1 of (a) shows the immunoblot of four HIV-1 p24-positive samples and their respective Coomassie-stained protein profiles. Gel 2 of (a) shows three representative HIV-1 p24-negative CVL in lanes 2, 4, and 5 of the immunoblot with their respective Coomassie-stained protein profiles shown above. (b) CVL samples of all 20 patients were also analyzed for the presence of HIV-1 gp41 antigen by WB and four representative WB data are shown: one HIV-1 gp41 antigen-negative CVL in lane 2 of the immunoblot, and three HIV-1 gp41 antigen-positive CVL samples in lanes 3, 4, and 5 of the immunoblot, respectively. The corresponding Coomassie-stained protein profiles of the samples are shown above.