FIG. 3.

Immunoblots probed with four mouse HIV-1 p24 monoclonal antibodies raised from separate clones. Equal aliquots of a CVL sample were loaded four times on a gel. After transferring the proteins the immunoblot was split into four sections and probed with an HIV-1 p24 mAb obtained from the following sources: (1) Abcam Inc., Cambridge, MA; (2) Cliniqa, Fallbrook, CA; (3) ZeptoMetrix, Buffalo, NY; and (4) New York Blood Bank, New York, NY. Identical bands in all four immunoblots confirmed the presence of HIV-1 p24 in the CVL.