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. 2009 Mar 28;25(3):249–259. doi: 10.1089/aid.2008.0096

FIG. 4.

FIG. 4.

Immunoblot of immunoaffinity column eluates probed with HIV-1 p24 and phosphotyrosine 4G10 mAbs. The samples loaded on the gel were as follows: lane 1 = molecular weight marker; lane 2 = HIV-1 p24 full length recombinant protein (positive control); lane 3 = untreated CVL sample; lane 4 = CVL sample treated with protein A + protein G treated to remove IgG; lane 5 = eluate from the immunoaffinity column in which the CVL sample was loaded; lane 6 = eluate from the control immunoaffinity column. The HIV-1 p24-positive band in lane 5 (chromatography column eluate) confirmed the presence of p24 antigen in the CVL. Two additional bands were noted in lane 5, one corresponding to 41 kDa and the other to 55 kDa. These are probably HIV 41 and 55 gag proteins that could be visualized only when 160 μl of CVL was loaded onto the column (a). (b) The WB results when the same immunoblot was reprobed with phosphotyrosine, recombinant 4G10 mAb.