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. 2008 Oct 10;10(10):1699–1712. doi: 10.1089/ars.2007.1999

FIG. 6.

FIG. 6.

AOPPs-induced ICAM-1 and VCAM-1 expression in HUVECs. HUVECs were incubated with indicated concentrations of AOPPs-HSA for 6 h (A) or with 200 μg/ml of AOPPs-HSA for indicated time (B). ICAM-1 and VCAM-1 mRNA expression was measured by real time RT-PTCR. Expression of ICAM-1 and VCAM-1 protein was detected by immunoblotting (C and D). (E) Representative immunofluorescent staining for ICAM-1 and VCAM-1 in HUVECs. (F and G) HUVECs were pretreated with the α-RAGE IgG, apocynin, c-SOD, U0126/SB203580, and SN50. AOPPs-induced expression of ICAM-1 (F) and VCAM-1 (G) were determined by immunoblotting. Data from three independent experiments are shown as mean ± SEM. ANOVA, p < 0.001 in A, B, C, D, F, and G; *p < 0.05 as compared with group treated with medium alone; #p < 0.05 vs. group without respective inhibitors. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).